M.Sc. (Hons) Biochemistry Syllabus

Semester I

  • COURSE CODE
    COURSE NAME
    CREDITS
  • JMBC-011

    Quantitative Analytical Methods:

    UNIT-I

    Introduction to Quantitative Analytical Methods.

    UNIT-II

    Errors, Precision & Accuracy in Analytical Methods.

    UNIT-III

    Classical methods in Analysis – Gravimetric, Volumetric – Neutralization titrations, complexometry & Iodometry.

    UNIT-IV

    Electrochemical Methods – Redox Titration, Potentiometry, Thermogravimetry, Coulometry, Voltametry.

    UNIT-V

    Colorimetry, Spectrophotometry

    UNIT-VI

    Chromatography – Adsorption, Partition, Gel filteration, Ion exchange.
    04
  • JMBC-012

    Applied Chemistry:

    UNIT-I

    Amino acids and Proteins: Structure and properties of amino acid and non-standard amino acids. Proteins: Classification, structure & properties. Biological significance of proteins

    UNIT-II

    Carbohydrate: Monosaccharides, Oligosaccharide, Polysaccharide, Glyco protein and its biological significance.

    UNIT-III

    Lipids: Classification, structure & properties of lipids, Properties of lipid Aggregates and its biological significance.

    UNIT-IV

    Nucleic Acid: Chemistry of Nucleic acid, Structure and composition of purine and pyrimidine, Nucleosides, Nucleotides, general composition of DNA & RNA. Biological significance of nucleic acid.

    UNIT-V

    Synthetic Polymers: Addition polymers, copolymers thermo-plastic and thermosetting polymers, Natural & synthetic rubbers.

    UNIT-VI

    Colour, Dye and Pigments: Constitution classification of dyes

    UNIT-VII

    Ionization of water, Equilibrium constants, pH scale, Weak acids and bases, Expression relating pH pK and buffer concentration application of pH, buffer in biological systems. Publication.
    04
  • JMBC-013

    Intermediary Metabolism-I:

    UNIT-I

    Bioenergetics: Energy & its- transformation in living systems ; Thermodynamic principles; concept of free energy changes in biochemical reactions, Electro-chemical gradient, energy rich compounds. Introduction to metabolism: methods to study metabolism.

    UNIT-II

    Carbohydrate metabolism: Introduction to digestion and absorption of carbohydrates. Catabolism of carbohydrates: Glycogenolysis, glycolysis, fermentation. TCA, ETS, Energy aspects, HMPS. Anabolism: Biosynthesis of structural polysaccharide. Eg.- peptidoglycon, chitin.Anabolic role of TCA cycle (anapleurotic reactions), gluconeogenesis, glycogenesis, glyoxalate cycle, regulation of carbohydrate metabolism.

    UNIT-III

    Lipid metabolism: Introduction to digestion and absorption, oxidation and biosynthesis of fatty acids. Phospholipids: energy aspects, importance and regulation. Biosynthesis of biologically important lipids: biosynthesis of sterols, glycolipids, prostaglandins, prostacyclins, thromboxins. Energy aspects, regulation and importance.

    UNIT-IV

    Oxidative phosphorylation: Introduction to electron transport, chain structure localization. Components, oxidative phosphorylation, ATP formation. Regulators, inhibitors and uncouplers.
    04
  • JMBC-014

    General Microbiology:

    UNIT-I

    Scope of microbiology and microorganism: History and scope of Microbiology General Characteristics, classification, morphology and reproduction of Bacteria, Mycoplasma, Chlamydia, Rickettsiae, and Actinomycetes.

    UNIT-II

    Fungi: General characteristics, classification, morphology and reproduction of Fungi.

    UNIT-III

    Algae: General characteristics, classification, morphology and reproduction of Algae

    UNIT-IV

    Protozoa: General characteristics, classification, morphology and reproduction of Protozoa.

    UNIT-V

    Control of Microorganism: Control of microorganisms by physical, chemical agents and growth curve.
    04
  • JMBC-015

    Molecular Cell Biology:

    UNIT-I

    Macromolecules: Biochemical and molecular aspects of living cells, Carbohydrates, Proteins and Nucleic acids. Cell fractionation procedure.

    UNIT-II

    Cell organelles: Molecular organization of cell organelles (structure and function), cell wall, plasma membrane (various models), endoplasmic reticulum, mitochondria, chloroplast, nucleus, etc.

    UNIT-III

    Cytoskeleton and Extra Cellular Matrix: Microtubules, intermediate filaments and micro filaments. ECM- definition, significance and biomolecules involved in ECM.

    UNIT-IV

    Cell growth and division: Cell cycle, mitosis, meiosis, DNA replication, apoptosis, cancer.

    UNIT-V

    Cell signaling and cell- cell interactions: Signal transduction, endocrine, paracrine and autocrine signalling, surface receptor mediated transduction, chemistry and function of signaling molecules.
    04
  • JMBC-016

    Statistics-I:

    Standard – deviation, Coefficient of variation, standard error of mean. Theory of Probability: equally likely, mutually exclusive events, definitions of probability, addition & multiplication theorems of probability & problems based on them. Normal & Binomial Distributions. Simple correlation & regression, Multiple- regression, Multiple & Partial- Correlation. Testing of Hypothesis: Concept of Hypothesis, Degrees of freedom, Level of significance. Type I & Type II errors. X 2, t, Z & F – tests. (Definition, applications & problems based on these tests).
    04
  • JMBC-161

    Biochemistry Lab I:

    Based on all theory papers.
    08
  • Total Credits
     
    32