B.Sc. (Hons) Biochemistry Syllabus

Semester IV

  • COURSE CODE
    COURSE NAME
    CREDITS
  • JBBC-401

    Hormone Biochemistry:

    UNIT-I

    Introduction: History, endocrine glands, hormones as chemical messengers,stimulus for hormone release: change in homeostasis, sensory stimulus and others.

    UNIT-II

    Cell signaling& Mechanism of Hormone action: Receptor study, Binding affinity, specificity, Scatchard plot and purification. G protein linked receptorfamily; Signal transduction pathways involving G-proteins, Adenylcyclases, Ca2+, Phosphoinositides, PI-3 Kinase, DAG, cAMP, cGMP, NO, Protein kinases (A,B,C,G). Receptor tyrosinekinase family- EGF receptor family, Insulin receptor family, & Cytokine/erythropoietin receptor family associated with non-receptor.

    UNIT-III

    Intra-cellular Receptors: Steroid hormone receptors, Thyroid hormone receptors. Sensitisation & Desensitization of receptors; Short term regulation &Long term regulation. Drugs and Toxins affecting cell signaling: Cholera toxin

    UNIT-IV

    Pituitary Hormones and Pancreatic Hormones: Insulin, Glucagon, Diabetes type I & II. Anterior Pituitary hormones- Growth hormone, Prolactin, POMC peptide family, LH, FSH, TSH; Posterior Pituitary: Vasopressin, Oxytocin.

    UNIT-V

    Endocrine disorders: Gigantism, Acromegaly, dwarfs, pigmies; Pathophysiology: Diabetes insipidus. Thyroid Hormone (include biosynthesis) Goiter, Graves disease, Cretinism, Myxedema, Hashimoto's disease.

    Books:

    • 1. Nelson, D.L. and Cox, M.M. (2005). Lehninger Principles of Biochemistry, W. H. Freeman & Com
    • 2. Widmaier, E.P., Raff, H. and Strang, K.T. (2008). Vander, Sherman, Luciano's Human Physiology, McGraw- Hill Higher Education.
    • 3. Darnell, J., Lodish, H. and Baltimore, D. (2008). Molecular Cell Biology, Scientific American Books
    04
  • JBBH-401

    Microbiology and Plant Pathology:

    UNIT-I

    General Microbiology: Introduction to microbial world, microbial nutrition. Virus: Discovery, physiochemical and biological characteristics; Classification; replication, lytic and lysogenic cycle; TMV virus. Economic importance; Symptoms, Transmission of diseases caused by viruses on plants. Bacteria: General characteristics, comparison of Archaebacteria and Eubacteria, Mycoplasma, bacterial cell structure, nutrition; reproduction: vegetative, asexual, sexual (conjugation, transformation, transduction), Economic importance.

    UNIT-II

    Applied microbiology: Microbial culturing technique (Isolation and Cultivation), culture media, and microbial growth, microbes used in agriculture, and industry, mycorrhizae.

    UNIT-III

    General account of plant pathogens: Historical development in Plant Pathology, general account of diseases (Symptoms and management) caused by plant pathogens e.g. Little Leaf of brinjal, tobacco mosaic virus, Stem rust and loose smut of wheat, White rust of crucifers, Early and late blight of potato, Red rot of sugarcane and Citrus canker.

    UNIT-IV

    Pathogen attack and defense mechanism: Physical and biochemical aspects. Plant disease epidemiology; Transmission and disease cycles, spread of plant pathogens and disease forecasting. Plant disease management: Chemical, biological.

    Books:

    • 1. Wiily, Sherwood & Woalverton, Prescott's Microbiology, McGrew, Hill International Edition.
    • 2. Pelezar, Chan & Krieg, Textbook of Microbiology, McGrew & Hill International Edition.
    • 3. Sharma, P.D., Plant Pathology, Rastogi Publication.
    04
  • JBCH-401

    Analytical Chemistry:

    UNIT-I

    Qualitative and Quantitative aspects of analysis: Sampling, evaluation of analytical data, errors, accuracy and precision, methods of their expression, normal law of distribution if indeterminate errors, statistical test of data; F, Q, and T test, rejection of data, and confidence intervals.

    UNIT-II

    Optical methods of analysis: Origin of spectra, interaction of radiation with matter, fundamental laws of spectroscopy and selection rules, validity of Beer-Lambert's law. UV-Visible Spectrometry: Basic principles of instrumentation (choice of source, monochromator and detector) for single and double beam instrument; Basic principle of quantitative analysis: estimation of metal ions from aqueous solution, geometrical isomers, keto-enol tautomers. Determination of composition of metalcomplexes using Job's method of continuous variation and mole ratio method. Infrared Spectrometry: Basic principles of instrumentation (choice of source, monochromator & detector) for single and double beam instrument; sampling techniques. Structural illustration through interpretation of data, Effect and importance of isotope substitution. Flame Atomic Absorption and Emission Spectrometry: Basic principles of instrumentation (choice of source, monochromator, detector, Choice of flame and Burner designs. Techniques of atomization and sample introduction; Method of background correction, sources of chemical interferences and their method of removal. Techniques for the quantitative estimation of trace level of metal ions from water samples.

    UNIT-III

    Electro analytical methods: Classification of electroanalytical methods, basic principle of pH metric, potentiometric and conductometric titrations. Techniques used for the determination of equivalence point. Techniques used for the determination of pKa values.

    UNIT-IV

    Separation Techniques: Solvent extraction: Classification and principle and efficiency of the technique. Mechanism of extraction: extraction by solvation and chelation. Technique of extraction: batch, continuous and counter current extractions. Qualitative and quantitative aspects of solvent extraction: extraction of metal ions from aqueous solution, extraction of organic species from the aqueous and non aqueous media. Chromatography: Classification and principle and efficiency of the technique. Mechanism of separation: adsorption, partition & ion exchange. Development of chromatograms: frontal, elution and displacement methods. Qualitative and quantitative aspects of chromatographic methods of analysis: IC, GLC, GPC, TLC and HPLC.

    Books:

    • 1. Vogel, Arthur I: A Test book of Quantitative Inorganic Analysis (Rev. by GH Jeffery and others) 5th Ed. The English Language Book Society of Longman
    • 2. Willard, Hobert H. et. al: Instrumental Methods of Analysis, 7th Ed. Wardsworth Publishing Company, Belmont, California, USA, 1988.
    • 3. Christian, Gary D; Analytical Chemistry, 6th Ed. New York- John Willy, 2004.
    • 4. Harris, Daniel C: Exploring Chemical Analysis, 2nd Ed. New York, W.H. Freeman, 2001.
    • 5. Khopkar, S.M. Basic Concepts of Analytical Chemistry New Age, International Publisher, 2009.
    • 6. SKoog, D.A. Holler F.J. and Nieman, T.A. Principles of Instrumental Analysis, Thomson Asia Pvt. Ltd. Singapore.
    • 7. Mikes, O. & Chalmes, R.A. Laboratory Hand Book of Chromatographic & Allied Methods, Elles Harwood Ltd. London.
    • 8. Ditts, R.V. Analytical Chemistry – Methods of separation.
    04
  • JBBC-451

    Hormone Biochemistry Practical:

    Based on theory paper of Hormone Biochemistry
    02
  • JBBH-451

    Microbiology and Plant Pathology Lab:

    Based on theory paper of Microbiology and Plant Pathology
    02
  • JBCH-451

    Analytical Methods in Chemical Analysis:


    • A. Quantitative Organic Analysis
      • a. Separate and identify the monosaccharide present in the given mixture (glucose & fructose) by paper chromatography.
      • b. Determine the pH of given aerated drinks and fruit juices and soaps.
      • c. Determination of dissolved oxygen in water.
      • d. Determination of biological oxygen demand.
      • e. Determination of chemical oxygen demand.
      • f. Estimation of Barium as barium sulphate by gravimetric analysis.
    • B. Qualitative Organic Analysis
      • a. Detection of elements
      • b. Detection of functional group
    02
  • Total Credits
     
    18