M.Sc. (Hons) Biochemistry Syllabus

Semester II

  • COURSE CODE
    COURSE NAME
    CREDITS
  • JMBC-023

    Intermediary Metabolism-II:

    UNIT-I

    Amino acid catabolism: Introduction to digestion and absorption, amino acid degradation pathways and their mode of degradation- Transamination, oxidative deamination, deamination and importance. Urea cycle, formation of excretory products, linking to TCA cycle and regulation.

    UNIT-II

    Amino acid biosynthesis: Overview, biosynthesis of essential amino acids tryptophan, tyrosine, valine, lysine. Biosynthesis of non-essential amino acids: glycine, serine, cystine, threonine, phenylalanine, histidine, praline, arginine

    UNIT-III

    Amino acid biosynthesis: Overview, biosynthesis of essential amino acids tryptophan, tyrosine, valine, lysine. Biosynthesis of non-essential amino acids: glycine, serine, cystine, threonine, phenylalanine, histidine, praline, arginine

    UNIT-IV

    Membranes- Transport phenomenon: Biomembranes, diffusion- passive and facilitated and active transport proceses. Mechanism of ATP synthase and its regulation, malate aspartate shuttle.
    04
  • JMBC-021

    Techniques in Biochemistry and Instrumentation:

    UNIT-I

    Chromatography: Adsorption, partition, exclusion, ion exchange, affinity, H.P.L.C., G.L.C., column.

    UNIT-II

    Biosensors: Types and uses.

    UNIT-III

    Centrifugation Techniques: Differential, zonal and density gradient, ultra, types of centrifuges and applications.

    UNIT-IV

    Nucleic Acid: Chemistry of Nucleic acid, Structure and composition of purine and pyrimidine, Nucleosides, Nucleotides, general composition of DNA & RNA. Biological significance of nucleic acid.

    UNIT-V

    Isolation and purification of DNA, RNA, plasmid DNA, sequencing of proteins and nucleic acids, chemical synthesis of nucleotides, competent cell preparation and transformation, DNA foot printing, DNA fingerprinting, PCR.

    UNIT-VI

    Immunological Techniques: RIA, ELISA, flow cytometry.

    UNIT-VII

    Radio isotopic and tracer techniques: Detection and measurement of isotopes (GM and scintillation counters), autoradiography.
    04
  • JMBC-022

    Enzymology:

    UNIT-I

    Introduction of enzymes: General properties and significance, classification and nomenclature. Terms and definition in enzymology, co-factors, coenzymes, active site concept, isoenzymes, allosteric enzymes, marker enzymes, ribozyme, abzyme, synzyme, extremozyme, therapeutic enzymes and immobilized enzymes and multienzyme complex.

    UNIT-II

    Enzyme kinetics: steady rate kinetics, Derivation of Michaelis-menten equation using steady state/equilibrium kinetics, plots of lineweaver- Burk, Hanes, Eadie- Hofster etc. Mechanism of bisubstrate and multisubstrate enzyme catalyzed reaction, Enzyme inhibitors, mechanism of enzyme action-lysozyme, chymotrypsin, and alcohol dehydrogenase.

    UNIT-III

    Regulation of enzyme activity: Allosteric model concerted and sequential, cooperativity. Feedback inhibition.

    UNIT-IV

    Enzyme Technology: Commercial production of enzymes, immobilization of enzymes, example of enzyme engineering, application of enzyme(therapeutic uses, analytical uses, manipulated uses etc.), uses of enzyme reactors.

    UNIT-V

    Isolation, purification and localization of enzymes. Various methods to estimate the enzyme activity.
    04
  • JMBC-025

    Computer Orientation:

    UNIT-I

    Information Concepts

    UNIT-II

    Computer Appreciation

    • a) Definition, Characteristics and Application of Computers
    • b) Computer Hardware, I/O Devices, Memory CPU
    • c) Software Concepts

    UNIT-III

    Operating System: DOS, Windows

    UNIT-IV

    Application Software: MS Word, MS Excel

    UNIT-V

    Computer Programming: Concepts of Algorithm & Flowchart, Introduction to 'C' Language. History, Input and Output Statements, Variables and Constants, Expressions and Operators, Control Statements, Branching Statements (if, if-else, nested if), Looping Statements (while, do-while, for-next), Functions and arrays.

    UNIT-VI

    Internet Concepts and Search Engine.
    04
  • JMBC-024

    Molecular Genetics

    UNIT-I

    Gene concept and Gene analysis: The gene and gene concept, cistron as a unit of genetic function. Prokaryotic genome, eukaryotic genome, viral genome, genetic code, central dogma including reverse transcription.

    UNIT-II

    Transcription: Synthesis of RNA, classes of RNA molecules, mechanism of transcription in prokaryotes and eukaryotes- RNA polymerases, promoter sequences for RNA polymerases, enhancers and silencers, transcription factors and initiation of transcription, basal transcription apparatus, elongation and termination of transcription. Post transcriptional modifications (mRNA processing reactions- 5' capping, polyadenylation, splicing).

    UNIT-III

    Translation: Introduction to protein synthesis (ribosomes, codon- anticodon interaction). Protein synthesis in prokaryotes and eukaryotes. Translational factors and their functions. Initiation, elongation and termination phases of translation. Comparison of protein synthesis in prokaryotes and eukaryotes. Post translational modification of proteins (protein folding and protein trafficking). Antibiotics as inhibitors of protein synthesis.

    UNIT-IV

    Gene regulation: Gene regulation in prokaryotes, transcriptional control (lac operon model, catabolite repression, tryptophan operon model). Regulation of gene expression in eukaryotes (transcriptional and translational controls). Gene regulation in plant cells.

    UNIT-V

    Mutation: Study of mutation at molecular level. Types of mutation (gene mutation and chromosomal abberations); Molecular basis of gene mutation, tautomeric shifts and abnormal base pairing (transitions and trans versions); Frame shift mutations (deletions or additions); Mutagenesis, physical and chemical mutagenic agents; DNA damage and DNA repair mechanisms.
    04
  • JMBC-026

    Statistics-II:

    Analysis of variance technique: Definition & assumptions, One way classification, two way classification with more than one observation per cell. Designs of experiments: Principles of Experimental- Design, Randomized Block Design (R.B.D), Latin Square Designs (L.S.D), Missing Plot Technique in R.B.D & L.S.D. Critical-difference (C.D) Split plot design. Factorial – Experiments: 22, 23, 32, & 33, factorial-designs. (Yates method of Analysis), 2 x 3 & 2 x 4 factorials. Durcan's Multiple Range Test. Newman's Kuel's Test Sampling techniques: Simple Random Sampling, Stratified Random Sampling & Systematic Sampling.
    04
  • JMBC-261

    Biochemistry Lab II:

    Based on all theory papers.
    08
  • Total Credits
     
    32