M.Sc. (Hons) Biochemistry Syllabus
Semester III
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COURSE CODECOURSE NAMECREDITS
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JMBC-033
Immunology & Medical Biochemistry:
UNIT-I
Fundamental Immunology: Basic terminology: Antigen, antibody, lymphokines, cytokines, hapten, adjuvants, immunogen, pathogen, epitopes, paratopes, etc. specific and nonspecific immune response, humoral and cell mediated immunity. Cells of adaptive immune response- B & T cell. Cells of innate immune response- macrophages, phagocytes, mast cells, dendritic cells, granulocytes, agranulocytes. Organ of immune system- 1.primary lymphoid organs 2.secondary lymphoid organs
UNIT-II
Generation of antibody diversity, clonal selection hypothesis definition idiotopes, idiotypes, allotypes, structure and function of M.H.C (both class 1&class 2), chemistry of antigen –antibody interaction, precipitation & agglutination.
UNIT-III
Structure and function of natural killer cells, superantigens, cells-mediated cytotoxicity (A.D.C.C), the complement system (both classical & alternative path ways).experimental assessment of A.D.C.C i.e. by C.M.L, M.L.R and graft vs. host reaction.
UNIT-IV
Primary B & T-cells immunodefciencies, auto immunity hypersensitivity, structure of t-cells receptor and comparison with antibody molecule.
UNIT-V
Techniques of immunology, immunoelectrophoresis (one dimensional and two dimensional), ELISA, RIA, Immunofluorecence, western immuno blotting, biochemistry of cancer & AIDS. Diagnostic enzymes (LDH, AST, ALT, CK, Troponin erc.)
04 -
JMBC-035
Plant Biochemistry:
UNIT-I
Photosynthesis: significance of photosynthesis, ultra structure of chloroplast, photosynthetic pigments. Light absorption phenomenon, Photosynthesis in C3 and electron transport, photophosphorylation: photo respiration, CAM.
UNIT-II
Bacterial Photosynthesis, photochemistry and electron transport and CO2 fixation.
UNIT-III
Nitrogen metabolism: Metabolism of N-compounds in plants, biological nitrogen cycle, nitrogenase structure and function, nitrate reduction, nitrification, denitrification, symbiotic and non symbiotic nitrogen fixation, Nif- genes- organization, function and regulation, Assimilation of fixed nitrogen by plants.
UNIT-IV
Plant Hormones: Definition of phyto hormones, Auxins, biochemistry and mode of action of Auxins, Gibberellin, Cytokinins and other natural growth hormones in plants (ethylene, abscissic acid ).
UNIT-V
Plant disease and defense mechanism: Biochemistry of bacterial and viral and fungal diseases, Micro and Macro nutrients deficiency in plants. (Biochemical role of inorganic ions in plants).
UNIT-VI
Secondary metabolism in plants: Phenolic metabolism shikimate, and phenyl propanoid pathways, flavonoids, lignins, and anthocyanins. Isoprenoid metabolism, terpenoids and carotenoids,
04 -
JMBC-031
Animal Biochemistry:
UNIT-I
Biochemistry of Blood and Body fluids-Biochemical composition of blood groups, path way of blood clotting, physiological function of blood, buffering action of blood, kinds other body fluids and their chemistry.
UNIT-II
Biochemistry of Blood tissue-Connective tissue, collagen, elastin muscle protein, muscle construction, lens proteins.
UNIT-III
Biochemistry of Fat in adipose tissue , steroids-structure and biochemical function cholesterol-Structure and biochemical synthesis, plasma lipids, transport of lipids, lipo-protein, chylomicrons, VLDL, HDL, LDL, and Lp (a), free fatty acids, non-esterified fatty acids.
UNIT-IV
Gastrointestinal physiology-Digestion of food ruminants and non-ruminants absorption of food, biochemical role of liver, bile acids and bile salt.
UNIT-V
Anti-oxidant-Generation of free radicals, damage produced by reactive-oxygen species (ROS), free-radicals, and scavenging enzyme system.
UNIT-VI
Animal Hormones-Origin, site of action, biochemical mechanism of hormone action, feedback mechanism of hormonal secretion.
04 -
JMBC-034
Nutritional Biochemistry:
UNIT-I
Nutritional aspects of carbohydrates- Biochemical functions the relative importance of different carbohydrates in diet, utilization of absorbed carbohydrates in the body, regulation of blood glucose level of blood dietary fibre and their biochemical effect in human nutrition.
UNIT-II
Nutritional aspects lipids- Fats in the body and food, biochemical function of fats, role of in diet, effect of trans fatty acids, blood lipids, transport and storage of lipids, role of liver in lipid metabolism, omega fatty acids.
UNIT-III
Nutritional aspects proteins- Nutritional significance of amino acids , specific function of some important amino acids, complementary value of proteins, methods of proteins (BV, NB, PER, NPR)
UNIT-IV
Role of vitamins minerals in health and disorders biochemical function of water detoxification.
04 -
JMBC-032
Bioinformatics:
UNIT-I
Introduction to Bioinformatics, philosophical, directional and application oriented background of bioinformatics.
UNIT-II
HGP – influence area in Bioinformatics, Application in different industries, and its Indian scenario, as a business, problem and future aspects.
UNIT-III
Information network – Internet, web Browser and address (NCBI, EBI etc).
UNIT-IV
Databases – information resources for Proteins and Genomics.
UNIT-V
Phylogenetic Analysis: Fundamental of Phylogenetic model, Phylogenetic tree interpretation – Paralogues and orthologues, Tree building and tree evaluation, Phylogenetic software.
UNIT-VI
Molecular structure Predication and visualization (X-ray crystallography/ NMR/ Bioinformatics).
UNIT-VII
Micro array data analysis.
04 -
JMBC-036
Plant Tissue Culture:
UNIT-I
Introduction to plant tissue culture: Historical developments and landmarks in Plant Tissue Culture. Organization of tissue culture laboratory, aseptic techniques, media formulation, clonal propagation vs tissue culture, Totipotency: growth, differentiation and morphogenesis in tissue culture.
UNIT-II
Micropropagation: Concept, various stages, organogenesis and somatic embryogenesis. Meristem culture: Meristem culture for mass and clonal propagation, production of pathogen free plants, application in forestry.
UNIT-III
Somatic hybridization: Isolation, purification and culture of protoplasts, protoplast fusion and somatic hybridization, identification and characterization of somatic hybrids/cybrids, its applications.
UNIT-IV
Secondary metabolites: Production of secondary metabolites by plant cell culture, hairy root culture, and biotransformation.
UNIT-V
Cell Lines: Cell line selection for resistance to herbicide, stress, insect and diseases.
UNIT-VI
Haploid culture: Tissue culture methods for haploid production & its applications.
04 -
JMBC-361
Biochemistry Lab III:
Based on all theory papers.
08 -
Total Credits32